Vogl

A. Wayne Vogl

Professor

Cell & Developmental Biology Research Group
PhD (University of British Columbia)

Email: wayne.vogl@ubc.ca

Office: 604-822-2395

Research Interests
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In my laboratory we are interested in the interaction between intercellular junctions and the cytoskeleton in cells within the context of tissues, and in determining how the two systems function together to generate complex remodeling events in epithelia. We use the seminiferous epithelium of the mammalian testis as an ideal model system for our studies because intercellular junctions are large, easy to visualize, and can be readily isolated. Moreover, all three major elements of the cytoskeleton (actin filaments, intermediate filaments and microtubules) are abundant and associated with junctions. Importantly, junctions and elements of the cytoskeleton together are associated with major epithelial remodeling events, such as sperm release, turnover of the blood-testis barrier, and the translocation of developing sperm cells, that occur during spermatogenesis. We currently are exploring two major hypotheses:

(1) changes in position of spermatids in the seminiferous epithelium are due to the microtubule-based transport of specialized actin-associated adherens junctions that occur in Sertoli cell regions attached to developing spermatid heads;

(2) actin-related tubular membrane structures, termed tubulobulbar complexes, that develop during junction disassembly in the seminiferous epithelium are sub-cellular machines responsible for internalizing intact junctions during sperm release and turnover of the blood-testis barrier.

We make extensive use of imaging techniques (electron microscopy, conventional fluorescence microscopy, and confocal microscopy) in our research.

Publications
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  1. Lyon, K., Adams, A., Piva, M., Asghari, P., Moore, E.D., Vogl, AW*. (2017) Ca2+ signaling machinery is present at intercellular junctions and structures associated with junction turnover in rat Sertoli cells. Biology of Reproduction 96:1288-1302
  2. Adams, A., Vogl, AW*. (2017) High resolution localization of Rab5, EEA1 and nectin-3 to tubulobulbar complexes in the rat testis. Anat. Rec. 300:1160-1170
  3. Sriram, A., Lyon, K.R.P., Ho, C.D., Huynh, N., Vogl, AW*. (2016) Actin disruption results in altered morphology of basal tubulobulbar complexes in rat seminiferous epithelium. Anat. Rec. 299(10):1449-55.
  4. Lyon K, Bosseboeuf E, Vogl AW*. (2015) A new model of tubulobulbar complex internalization during junction remodeling in the seminiferous epithelium of the rat testis. Biol. Reprod 93(1):12, 1-11. [IF 3.32].
  5. Clark TE, Lillie MA, Vogl AW*, Shadwick RE, Gosline JM. (2015) Mechanical contribution of lamellar and interlamellar elastin along the mouse aorta. Biomechanics (Accepted for publication). [IF 2.75].
  6. Lo KY, Visram D, Vogl AW, Shen CLJ, Guttman JA. (2015) Morphological analysis of Franscicella novicida epithelial cell infections in the absence of functional FipA. Cell and Tissue Research (Accepted for publication). [IF 3.56].
  7. Vogl AW*, Lillie MA, Piscitelli MA, Goldbogen JA, Pyenson ND, Shadwick RE. (2015) Stretchy nerves: essential component of an extreme feeding mechanism in rorqual whales. Current Biology 25:R360-1. [IF 9.92].
  8. Goldbogen JA, Shadwick RE, Lillie MA, Piscitelli MA, Potvin J, Pyenson ND, Vogl AW*. (2015) Using morphology to infer physiology: case studies on rorqual whales (Balaenopteridae) Canadian Journal of Zoology-Revue Canadienne De Zoologie (Accepted for publication). [IF 1.35].
  9. Morell M, Lenoir M, Shadwick RE, Jauniaux T, Dabin W, Begeman L, Ferreira M, Maestre I, Degollada E, Hernandez-Milian G, Cazevieille C, Fortuño J, Vogl W, Puel J, André M. (2014) Ultrastructure of the Odontocete Organ of Corti: Scanning and Transmission Electron Microscopy. Journal of Comparative Neurology 523:431-48. [IF 3.51].
  10. Gilibili RR, Vogl AW, Chang TKH, Bandiera SM. (2014) Characterization and cellular localization of cytochrome P450 and related enzymes in adult rat testis and down-regulation by estradiol and bisphenol A. Toxicological Sciences 140:26-39. [IF 3.85].
  11. MacLeod G, Shang P, Booth GT, Mastropaolo LA, Manafpoursakha N, Vogl AW, Varmuza S. (2014) PPP1CC2 can form a kinase/phosphatase complex with the testis specific proteins TSSK1 and TSKS in the mouse testis. Reproduction 147:1-12. [IF 3.14].
  12. De Asis MA, Pires M, Lyon K, Vogl AW*. (2014) A network of spectrin and plectin surrounds theactin cuffs of apical tubulobulbar complexes in the rat. Spermatogenesis (In Press – published online – 2013).
  13. Quaroni L, Christensen CR, Chen B, Vogl W,Monsalve M. (2013) Detection of protein structure of frozen ancient human remains recovered from a glacier in Canada using synchrotron-Fourier Transform Infared (FTIR). Microsc. Microanal 19:565-575. [IF 1.76].
  14. Du M, Young JS, De Asis M, Chan A, Cipollone J, Roskelley C, Nicolls P, Stanton P, Takai Y, Vogl AW*. (2013) A novel subcellular machine responsible for basal junction turnover in the seminiferous epithelium. Biol. Reprod 88:60:1-17. [IF 3.45].
  15. Shadwick RE, Goldbogen JA, Potvin J, Pyenson ND, Vogl AW. Novel muscle and connective tissue design enables hyper-extensibility and controls engulfment volume in lungefeeding whales. Journal of Experimental Biology 216:2691-2701. [IF 3.00].
  16. Lillie MA, Piscitelli MA, Vogl AW, Gosline JM, Shadwick RE. (2013) Cardiovascular design in fin whales: High-stiffness arteries protect against adverse pressure gradients at depth. Journal of Experimental Biology 216:2548-2563. [IF 3.00].
  17. Warner A, Xiong G, Qadota H, Rogalski T, Vogl AW, Moerman DG, Benian GM. (2013) CPNA-1, a copine domain protein, is located at integrin adhesion sites, and is required for myofilament stability in Caenorhabditis elegans. Mol Biol Cell 24:601-616. [IF 4.55].
  18. Nicholls PK, Harrison CA, Rainczuk E, Vogl AW, Stanton PG. (2012) Retinoic acid promotes Sertoli cell differentiation and antagonizes activin-induced proliferation. Cell. Endocrinology 377:33-43. [IF 4.24].
  19. Young JS, Takai Y, Kojic KL, Vogl AW*. (2012) Internalization of adhesion junction proteins and their association with recycling endosome marker proteins in rat seminiferous epithelium. Reprod 143:1-12. [IF 3.55].
  20. Young JNS, Vogl AW* (2012) Focal adhesion proteins zyxin and vinculin are co-distributed at tubulobulbar complexes. Spermatogenesis 2(1):1-6.
  21. Pyenson ND, Goldbogen JA, Vogl AW, Szathmary G, Drake R, Shadwick RE. (2012) Discovery of a sensory organ that coordinates lunge-feeding in rorqual whales. Nature 485:498-501. [IF 41.4].
  22. Choi J, Vogl AW, Kronstad JW. (2012) Regulated expression of cyclic AMP-dependent protein kinase A reveals an influence on cell size and the secretion of virulence factors. Microbiol 85:700-715. [IF 4.96].
  23. Young JS, de Asis M, Guttman J, Vogl AW*. (2012) Cortactin depletion results in short tubulobulbar complexes and spermiation failure in rat testes. Biology Open 1(8):1-9.
  24. Arena ET, Auweter SD, Antunes LCM, Vogl AW, Han J, Guttman JA, Croxen MA, Menendez A, Borchers CH, Finlay BB. (2011) The deubiquitinase activity of the Salmonella pathenogenicity island 2 effector, SseL, prevents accumulation of lipid droplets. Immun 79:4392-4400. [IF 4.16].
  25. Ruetz T, Vogl AW, Guttman JA. (2011) Detailed examination of cytoskeletal networks within EPEC pedestals. Rec 295:201-207. [IF 1.34].
  26. Christensen CR, Jackson M, Zhoa J, Vogl, AW, Monsalve MV. (2011) Synchrotron mid-infrared analysis of the Kwaday_ Dan Ts’Inchi ancient remains found in a glacier in Canada. Yearbook of Mummy Studies 1:115-119.
  27. Warner A, Oadota H, Benian GM, Vogl AW, Moerman DG. (2011) The C elegans paxillinortholog, PXL-1, is required for pharyngeal muscle contraction and for viability. Mol Biol Cell 22:2551-2563. [IF 4.94].
  28. Donohue E, Tovey A, Vogl AW, Arns S, Sternberg E, Young R, Roberge M. (2011) Inhibition of autophagosome formation by the benzoporphyrin derivative verteporfin. Biol. Chem 286:7290-7300. [IF 4.77].
  29. Forgione N, Vogl AW, Varmuza S. (2010) Loss of protein phosphatase 1cg leads to impaired spermatogenesis associated with defects in chromatin condensation and acrosome development: An Ultrastructural analysis. Reproduction.139:1021-9. [IF 3.05].
  30. Joe AW, Yi L, Even Y, Vogl AW, Rossi FM. (2009) Depot-specific differences in adipogenic progenitor abundance and proliferative response to high fat diet. Stem Cells 27:2563-2570. [IF 7.75].
  31. Menendez A, Arena ET, Guttman JA, Thorson L, Vallance BA, Vogl AW, Finlay B. (2009) Salmonella infection of gallbladder epithelial cells drives local inflammation and injury in a model of acute typhoid fever. Infect. Dis. 200:1703-13. [IF 5.86].
  32. Young JS, Guttman JA, Vaid KS, Shahinian H, Vogl AW*. (2009) Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis. Reprod 80:162-74. [IF 3.3].
  33. Young JS, Guttman JA, Vaid KS, Vogl AW*. (2009) Cortactin (CTTN), N-WASP (WASL) and clathrin (CLTC) are present at podosome-like tubulobulbar complexes in the rat testis. Reprod 80:153-61. [IF 3.3].
Further publications can be found here.
Books
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Drake, R, Vogl, AW, Mitchell, A, R Tibbitts, and P Richardson (2007) Gray’s Atlas of Anatomy. Elsevier ISBN 978-0-443-06721-1

Drake, R, Vogl, AW and Mitchell, A (2008) Dorland’s/Gray’s Pocket Atlas of Anatomy. Elsevier ISBN 978-0-443-06761-7

Drake, R, Vogl, AW and Mitchell, A (2019) Gray’s Anatomy for Students. 4th edition. Elsevier ISBN 978-0-323-39304-1

Drake, R, Vogl, AW and Mitchell, A (2020) Gray’s Anatomy for Students Flash Cards. 4th edition. Elsevier ISBN: 978-0-3236-3917-0

Drake, R, Vogl, AW and Mitchell, A (2017) Gray’s Basic Anatomy. 2nd edition.
Elsevier ISBN 978-0-323-47404-7

Invited Reviews
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  1. Adams, A, Sriram, A., Vogl, AW*. (2018) “Internalization of ‘intact’ intercellular junctions in the testis by clathrin/actin-mediated structures – Tubulobulbar complexes” Anat. Rec. 301:2080-2085
  2. Vogl, AW*, Lyon, K., Adams, A, Piva, M. and Nassour, V. (2017) “The endoplasmic reticulum, calcium signaling and junction turnover in Sertoli cells” Reproduction 155 R93-R104
  3. Hess, Rex A., Vogl AW*. (2015) “Sertoli cell anatomy and cytoskeleton” Sertoli Cell Biology, 2nd edition. M.D. Griswold edt. Elsevier, Amsterdam, Netherlands
  4. Vogl AW*, Young JS, Wang E, Du M. (2014) “Novel clathrin/actin-based endocytic machinery associated with junction turnover in the seminiferous epithelium” Seminars in Cell and Developmental Biology. [IF 265].
  5. Vogl AW*, Young JS, Du M (2013) “New insights into the role of tubulobulbar complexes in sperm release and turnover of the blood-testis barrier” Rev. Cell and Mol. Biol. 303: 319-354. [IF 4.522].
Images
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Stereoscopic confocal image of sertoli cell and spermatids labelled for tubulin and actin.